Meet Angie Estrada: Amphibian Ambassador

We are a small part of a much larger global effort called the Amphibian Ark program working to coordinate and support global efforts to prevent amphibian extinctions. Learn more about other cool amphibian conservation work at the AArk ambassador’s page and like their Facebook page.

Mission Critical: Amphibian Rescue

This award-winning documentary featuring our race to find a cure for a deadly amphibian disease and to build an amphibian ark in Panama is now available for FREE.  Watch the trailer below and download the full feature if you would like to see more on the itunes store for a limited time only.

CLICK HERE to download the full episode of Smithsonian Networks Mission Critical: Amphibian Rescue film. FREE FOR A LIMITED TIME ONLY!

Rescue Project Successfully Breeds Endangered Frog Species

 

Limosa harlequin frog (Atelopus limosus) baby on a U.S. quarter.

Limosa harlequin frog (Atelopus limosus) baby on a U.S. quarter. (Photo by Brian Gratwicke, Smithsonian Conservation Biology Institute)

The limosa harlequin frog (Atelopus limosus), an endangered species native to Panama, now has a new lease on life. The Panama Amphibian Rescue and Conservation Project is successfully breeding the chevron-patterned form of the species in captivity for the first time. The rescue project is raising nine healthy frogs from one mating pair and hundreds of tadpoles from another pair.

“These frogs represent the last hope for their species,” said Brian Gratwicke, international coordinator for the project and a research biologist at the Smithsonian Conservation Biology Institute, one of six project partners. “This new generation is hugely inspiring to us as we work to conserve and care for this species and others.”

Nearly one-third of the world’s amphibian species are at risk of extinction. The rescue project aims to save priority species of frogs in Panama, one of the world’s last strongholds for amphibian biodiversity. While the global amphibian crisis is the result of habitat loss, climate change and pollution, a fungal disease, chytridiomycosis, is likely responsible for as many as 94 of 120 frog species disappearing since 1980.

Between its facilities at the Smithsonian Tropical Research Institute in Gamboa, Panama, and the El Valle Amphibian Conservation Center in El Valle, Panama, the rescue project currently cares for 55 adult limosa harlequin frogs of the chevron-patterned form and 10 of the plain-color form. The project has had limited success breeding the plain-color form of this species, and has successfully bred other challenging endangered species, including crowned treefrogs (Anotheca spinosa), horned marsupial frogs (Gastrotheca cornuta) and toad mountain harlequin frogs (A. certus).

Each species requires its own unique husbandry to thrive and breed. The project’s animal care team and scientists learn husbandry techniques as they work with a limited number of individuals. Jorge Guerrel, conservation biologist at the Smithsonian Tropical Research Institute, arranged rocks in the breeding tank to create the submerged caves that appear to be the preferred egg deposition sites for limosa harlequin frogs. Like other Atelopus species, tadpoles require highly oxygenated, gently flowing water between 22 and 24 degrees Celsius. The tadpoles’ natural food is algal film growing on submerged rocks, which Guerrel and his colleagues re-created by painting petri dishes with a solution of powdered spirulina algae, then allowing it to dry.

The mission of the Panama Amphibian Rescue and Conservation Project is to rescue amphibian species that are in extreme danger of extinction throughout Panama. The project’s efforts and expertise are focused on establishing assurance colonies and developing methodologies to reduce the impact of the amphibian chytrid fungus so that one day captive amphibians may be reintroduced to the wild. Current project partners include Cheyenne Mountain Zoo, Houston Zoo, Smithsonian’s National Zoological Park, Smithsonian Tropical Research Institute and Zoo New England.

Lindsay Renick Mayer, Smithsonian’s National Zoo

Frog Love on Valentine’s Day

This Valentine’s Day we asked some of the rescue project’s researchers why they love frogs. Here’s what we got back from a few biologists at the Smithsonian’s National Zoo. The common link? A life-long love of wildlife.

Matt Evans, biologist, Smithsoinan's National Zoo

Matt Evans, biologist, Smithsonian

“My favorite childhood memories revolve around my mother taking me down to the nearest stream and letting me get dirty playing with frogs and salamanders. I loved it so much, I get to do that for a living now! The diversity within all amphibians still amazes me as there is so much we still have  to learn. I consider being able to work on projects, which may help save frogs from extinction, to be the absolute coolest part of my job.”

Ed Smith, biologist, Smithsonian's National Zoo

Ed Smith, biologist, Smithsonian

“Since childhood it was snakes, not frogs, that were the focus of hours spent searching woodlands, streams and ponds. Unlike other more noticeable  creatures, snakes are decidedly unapparent; unless they happen to be eating a frog! Although, the thrill of finding a snake by following the plaintive screams of a leopard frog never diminished, what increased was my interest in the gradually disappearing prey. So, thanks in part to the appetite of a few alluring garter snakes the equally marvelous world of frogs, toads, salamanders, sirens, and hellbenders opened to me and now inform and enhance days at work and in the field.”

Brian Gratwicke, rescue project international coordinator

Brian Gratwicke, rescue project international coordinator

“I fell in love with frogs growing up as a child in Zimbabwe. There was something exhilarating about discovering the translucent, musical jewels that are responsible for the familiar nocturnal soundtrack of my childhood. Unlike any computer games or TV shows, I will carry those first memories of catching frogs around a pond with me for the rest of my life. For me, seeing frogs in the wild stirs emotions of wonder and discovery, and they are accessible to everyone who is willing to take a little extra effort to open their eyes and look.”

–Brian Gratwicke, rescue project international coordinator

Genetic Matchmaking Saves Endangered Frogs

Marsupial frog

The casque headed tree frog (Hemiphractidae: Hemiphractus fasciatus), is one of 11 species of highest conservation concern now being bred in captivity in Panama. Females carry eggs on their backs where the young complete development hatching out as miniature frogs. DNA barcoding data suggest that populations of H. fasciatus may comprise more than one taxonomic group.

What if Noah got it wrong? What if he paired a male and a female animal thinking they were the same species, and then discovered they were not the same and could not produce offspring? As researchers from the Panama Amphibian Rescue and Conservation Project race to save frogs from a devastating disease by breeding them in captivity, a genetic test averts mating mix-ups.

At the El Valle Amphibian Conservation Center, project scientists breed 11 different species of highland frogs threatened by the chytrid fungus Batrachochytrium dendrobatidis, which has already decimated amphibian populations worldwide. They hope that someday they will be able to re-release frogs into Panama’s highland streams.

Different frog species may look very similar.

“If we accidentally choose frogs to breed that are not the same species, we may be unsuccessful or unknowingly create hybrid animals that are maladapted to their parents’ native environment,” said Andrew J. Crawford, research associate at the Smithsonian Tropical Research Institute in Panama and professor at Colombia’s Universidad de los Andes. Crawford and his colleagues make use of a genetic technique called DNA barcoding to tell amphibian species apart. By comparing gene sequences in a frog’s skin cells sampled with a cotton swab, they discover how closely the frogs are related.

The Smithsonian Tropical Research Institute, headquartered in Panama City, Panama, is a unit of the Smithsonian Institution. The Institute furthers the understanding of tropical nature and its importance to human welfare, trains students to conduct research in the tropics and promotes conservation by increasing public awareness of the beauty and importance of tropical ecosystems.

Beth King, Smithsonian Tropical Research Institute

Photo by Edgardo Griffith.

Frog Poetry and the Washington Post

Washington Post

On Dec. 30, the Washington Post ran a front-page story about the rescue project.

The year ended on a high note for the Panama Amphibian Rescue and Conservation Project. William Booth, a science writer for the Washington Post, joined rescue project researchers on a field expedition and his story about the rescue project came out on the front page of the Post on Dec. 30. The story inspired one reader, Tim Torkildson, to share a lovely poem about frogs and the disease that is wiping them out.

Booth also did this NPR interview about his recent trip to Panama.

If you saw the story and are interested in making a donation to the rescue project, please follow this link to the National Zoo’s website.

THE FROG
by Tim Torkildson

The frog is an amphibian
Who thrives most ev’rywhere,
From the dry Namibian
To just off ol’ Times Square.
The ones who have a bumpy skin,
With warts and pits and nodes,
Are the closest Phylum kin;
We simply call them toads.
The bullfrogs in the early spring
give ponds reverberation
With their raucous verbal fling,
Attempting procreation.
The have a courtship ritual
that’s called, I think, amplexus,
Which gives them fits conniptual
Between the two odd sexes.
A little boy will manage to
Corral a tadpole, yes,
And give it quite a slimy view
Right down his sister’s dress!
And did you know the urine from
a pregnant lady will
cause some frogs to lay a scum
of eggs, with no male thrill?
And so they’re useful critters,
As the French will tell you so;
Their legs taste good in fritters,
Are mistaken for turbot.
And what of cane toads, mind you,
Where, if you lick the skin,
The psychedelics blind you
To sorrow, grief and sin?
But frogs, those little gargoyles,
Which are funny in cartoons,
Are engaged in lethal broils
That leaves their lives in ruins.
A fungus known as “Bd” kills
The frogs down in Belize,
Then jumps the valleys and the hills
So others it may seize.
The Costa Rica Golden Toad
Is now extinct, alack.
More are headed down that road,
Since habitat is slack.
Toxins give some frogs three legs,
Which doesn’t help them jump.
Instead they are like clumsy kegs
Who in the water flump.
Scientists preserve some frogs
In habitats in labs.
Dressed in their starched, stiff white togs
They keep meticulous tabs.
To save the frogs, oh please donate
A dollar or a yuan,
So the polliwog birth rate
Will someday be a shoo-in!

Using genetic tools to understand and manage chytridiomycosis

Lowland leopard frog (Lithobates yavapaiensis)

The lowland leopard frog is the focus of SCBI postdoc Anna Savage's work looking at the relationship between genes and chytrid resistance.

Chytridiomycosis, the disease wiping out frog species around the world, was described in 1999 by a team of researchers at the Smithsonian and the University of Maine. Today, in addition to creating an insurance population for various Panamanian species, the Smithsonian Conservation Biology Institute aims to find a way to manage this disease. One of the most promising solutions to stopping the killer may rest in genetics. SCBI scientists are looking to identify genes within frogs that provide resistance to the pathogen or that make them more susceptible to it.

Researchers pursuing this approach are optimistic that genetics could provide different answers than those offered by probiotics, which SCBI is also pursuing. The genetic approach is one based on natural selection.

“If you look across species, including humans, diseases rarely wipe out an entire species without any evidence of resistance, regardless of how virulent they are,” said Anna Savage, an postdoctoral fellow in SCBI’s Center for Conservation and Evolutionary Genetics studying the genetics of chytrid. “Immune systems are so complex that there’s a strong possibility for the development of resistance. The probability that a species develops no resistance to a disease is rather small.”

Genetics research within the last decade has identified frogs with varying degrees of resistance to chytrid. Savage is focusing her research on the lowland leopard frog (Lithobates yavapaiensis), which exhibits an intermediate level of resistance to the pathogen. Some individuals demonstrate resistance while others of the same species die if infected with chytrid. The identification of genetic variations between the individuals holds the potential of being the answer between life and death. One of the new frontiers for genetic research is the examination of a frog species’ immune system genes.

“If we can identify the genes responsible for resistance, we can breed the animals in captivity to ‘spread’ this genetic resistance and give adaptation a headstart,” Savage said. “This area of research holds the potential for creating populations of self-sustaining frogs that confer resistance to the rest of the population through reproduction.”

Anna Savage with bullfrog

Savage's research could hold the key to putting a stop to a deadly pathogen killing frogs around the world.

In order to understand a frog’s response to chytrid, the focus must be broadened to understanding how immune system genes interact with one another and which ones are being expressed during resistance. Savage is using this approach to understand chytrid susceptibility in lowland leopard frogs that are being raised at the Smithsonian’s National Zoo. This approach could potentially help scientists identify genes responsible for chytrid resistance. While this type of research shows considerable potential, researchers are only just beginning to scratch the surface.

“Disease outcomes from chytridiomycosis can depend on several factors,” said Brian Gratwicke, SCBI wildlife biologist and Panama Amphibian Rescue and Conservation Project international coordinator.  “Some frogs appear to have innate resistance to the disease and produce antimicrobial peptides from glands in their skin. It is possible that some might acquire resistance through exposure, while other frogs have beneficial anti-fungal skin bacteria or a behavioral preference for temperatures that are bad for the fungus. As the fight against chytrid continues, we remain hopeful that the answers are out there and that Dr. Savage’s work will give us some insight into how this system really functions.”        

–Will Lazaro, Smithsonian’s National Zoo

(Frog photo by Jared Grummer; photo of Anna Savage by Dennis Caldwell)

Things we are thankful for: Amphibian baby boom at EVACC and Summit Zoo

Reflecting on our achievements this last year, we would like to thank you, our generous supporters. You have provided moral support; social media likes and retweets generously; more than 40 of you have given us your time to help us save frogs; and even more have sacrificed your hard-earned money to help us achieve this important mission. Thank you! In particular I would like to recognize the extraordinary dedication and above-and-beyond service from our devoted conservation staff seen above proudly displaying the the captive-bred products of their hard work, including a second generation of captive-bred frogs at EVACC, and our first grown-up generation of captive-born frogs at the Summit Zoo. We salute you all.

Brian Gratwicke – International project coordinator for the Panama Amphibian Rescue and Conservation Project, Smithsonian Conservation Biology Institute

Food for Frogs at EVACC: What it takes to raise the inverts served for dinner

Big frog, little frog, golden frog, marsupial frog, endangered frog, and common frog; what do all of those guys have in common? They all need to eat.  There is no call-up home delivery in Panama, so here in El Valle at EVACC, we have had to roll up our sleeves and become invertebrate breeders (adding to an eclectic list of “other skills” needed in this particular conservation project).

Lisandro Vasquez

Lisandro Vasquez, rolling up his sleeves in the cricket room at EVACC.

The thing that most people do not think about when thinking about an amphibian conservation project is food for the animals. It would be incredibly difficult to have a successful breeding/conservation project without being able to feed the subjects at hand. At EVACC we think about insects, and other invertebrates, on a very regular basis. And we think about what they eat, in a captive setting, just as much.

ingredients

Some of the ingredients that go into preparing insect diets.

What do our frogs eat?  We have quite a few different species we look after, so we have quite a few invertebrates to offer to them.  For the most part we delineate food items to specific species based on food size, and the mode in which the frog eats.  The smallest food we have to offer is springtails, from the insect order Collembola.  They go through simple metamorphosis, and the different size nymphs can be sifted and fed to different size amphibians.

Springtail colony

Springtail colony.

Working our way up through the sizes we have the two different kinds of fruit flies; Drosophila melanogaster and Drosophila hydei.  The two fruit flies at EVACC do not fly and the smaller one, D. melanogaster, does not have wings. They have both been genetically modified to possess these traits. Fruit flies, with vitamin powders, are fed alternately to our diurnal species, and to some of the nocturnal species at different stages in their life. These two species of Drosophilago go through complete metamorphosis.

Drosophila melanogaster in fly cup

Drosophila melanogaster in fly cup.

Drosophila hydei colony

Drosophila hydei colony.

Domestic crickets (Acheta domesticus) make up a large portion of the diet for many species at EVACC.  This captive food colony requires quite a bit of time and space, but well worth it.  This insect goes through incomplete or simple metamorphosis as well, making it a food item for the smallest of frogs to large ones, as well.

cricket box

Cricket box crawling with one-month-old nymphs.

Cricket breeding room

Cricket breeding room, boxes upon boxes of crickets stacked up at EVACC.

Woodlice, sow bugs, or roly-poly bugs are also on the menu at EVACC. These invertebrates are not insects, but a crustacean from the family Oniscidea.  Leaf litter frogs love these calcium-packed treats.

Woodlice

Woodlice at EVACC.

The super worm (Zophobas morio) is a larva of a species of darkling beetle. Only the larvae are fed out to amphibians, as the adult are not preferred foods for frogs. This insect goes through complete metamorphosis.

We also have a colony of earthworms. Our colonies are not thriving at the moment, so most of the time we harvest from our own backyard. The other food item that we are currently not breeding, but do feed out, is the Neoconocephalus saturatus, a type of cone-headed katydid. We rely on a local family to help us out by collecting these katydids for us.

Last, but certainly not least, is the newest food item on the menu at EVACC; Blaberus discoidalis, a very large cockroach.

Cockroach

Cockroach on the menu.

Larvae pupa and adult stages

Larvae, pupa, and adult stages of Zophobas morio

Heidi Ross, director of El Valle Amphibian Conservation Center

(All photos courtesy of EVACC)

Rescue project partner Houston Zoo wins grant to continue work on amphibian biodiversity in Borneo

Brown bullfrog (Kaloula baleata)

This brown bullfrog is one of a number of species that Houston Zoo and partners will be able to continue studying in Borneo thanks to this grant. (Photo courtesy of Houston Zoo)

The Houston Zoo and our partners at Hutan, Cardiff University and the Danau Girang Field Center (DGFC) were recently awarded a Conservation Endowment Fund (CEF) grant from the Association of Zoos and Aquariums (AZA).

The grant will allow the Zoo and our partners to build on the amazing work for the conservation of biodiversity that has been accomplished in Sabah in Borneo by Hutan and DGFC over the last few decades.

The primary threats in the area are the loss of primary and secondary forests to oil palm plantations. In 2008, Australian amphibian conservation biologist Dr. Graeme Gillespie began to work with Hutan and DGFC to include amphibians in their research and conservation programs.

Since 2008 they have intensively sampled the Lower Kinabatangan Wildlife Sanctuary and surrounding oil palm plantations to assess the amphibian fauna of the area. Results of the first phase were published in Biological Conservation earlier this year (Gillespie et al. 2012 152 (2012) 136–1440).

This grant will specifically address some of the questions relating to the value of the secondary forests for amphibian biodiversity by increasing the sampling done in primary areas. Once the dataset is assembled, we will be able to use this information to make additional recommendation for forest management.

Brian Hill, Houston Zoo