Tag Archives: Atelopus limosus

Following reintroduced frogs

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The Panama Amphibian Rescue and Conservation Project completed the first reintroduction trial of the Limosa Harlequin frog (Atelopus limosus) in 2017, and our findings were published in the journal Frontiers in Amphibian and Reptile Science. Reintroducing a species comes with a lot of unknowns and questions, just a few are: Where will the frogs go? What is their life like outside of a terrarium? Will they become infected with amphibian chytrid fungus? The purpose of this first reintroduction trial was to begin to unravel some of these questions so the researchers could adapt their strategies and improve the odds of the frogs in the wild.

We were able to get a detailed life of the frogs post-reintroduction by radiotracking the frogs and checking in on them daily. We found that when frogs were provided a 30-day acclimation period in a predator-free rainforest mesocosm, their probability of survival significantly increased and they did not disperse as far as the hard-released animals. We know from other studies that more movement can increase the likelihood of predators finding animals, and that likely happened in this study too. Frogs that were released without radio transmitters were 44x less likely to be reencountered during stream surveys (finding a frog in the rainforest isn’t easy!). We were able to follow the lives of these frogs in the wild for up to 56 days after release and developed a method that can use both radio-tracked animals and non-radiotracked animal encounters to estimate survivorship by assigning different detection probabilities in the model.

We observed a couple predation events of reintroduced frogs and some became infected with amphibian chytrid fungus. However, we learned a lot and has no shortage of questions to continue researching to get this species (and others in Panama) back into the wild.

Read the open access paper here:
Klocke, B., Estrada, A., Mataya, M., Medina, D., Baitchman, E., Belden, L., Guerrel, J., Evans, M., Baughman, J. and Connette, G., Illueca, E., Ibáñez, R., Gratwicke, B. (2023) Movement and survival of captive-bred Limosa Harlequin frog (Atelopus limosus) released into the wild. Frontiers in Amphibian and Reptile Science, 1, p.1205938. https://doi.org/10.3389/famrs.2023.1205938 

by Blake Klocke

Frog Friday: Limosa Harlequin Frog

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Atelopus limosus - Limosa Harlequin Frog

Cute, crafty, and brightly patterned with yellow and black chevrons! The Limosa harlequin frog is a terrestrial species endemic to Central Panama that live in moist, tropical Atlantic forests. In the wild, you could once spot this species among the dark rocks or mossy patches on the banks of streams. It is here, in the fast-flowing rainforest streams that enthusiastic males call and use their characteristic hand-waving behavior to signal females or warn competing males to steer clear. If successful, a male attracts a female and embraces her in a behavior known as amplexus. They laytheir eggs in fast-flowing streams for good reasons. The shallow, swiftly flowing water protects the eggs and tadpoles from potential predators and once they emerge as tadpoles the tadpoles cling tightly to rocks using the suction disks on their bellies while they graze on diatoms and other algae that grow on stones in these clear, oxygen-rich environments.

This species is listed as Endangered on the IUCN Red List. Atelopus frog species’ have been hit hardest by the growing chytridomycosis epidemic. While deforestation of habitats for agricultural use and infrastructural development, stream sedimentation, and water pollution are also serious threats to Atelopus limosus, much of their range is in the Chagres National Park and scientists attribute the recent disappearances of this species to the emergence of amphibian chytrid fungus. Thankfully, the Panama Amphibian Rescue and Conservation Project has brought the endangered species into captivity and successfully hosts a very fruitful breeding population – no small feat at all!

Did you know? The limosa harlequin frog has two color forms; the brown form with a yellow nose and yellow fingertips mostly found at lowland sites, while the green/yellow form with black chevrons is normally found in the highlands. If you’d like to get a look at one of these amazing creatures, you can see them on exhibit at the Punta Culebra Nature Center’s Fabulous Frogs of Panama!

by Dara Wilson, Panama Amphibian Rescue and Conservation Project Volunteer

Rescue Project Successfully Breeds Endangered Frog Species

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Limosa harlequin frog (Atelopus limosus) baby on a U.S. quarter.

Limosa harlequin frog (Atelopus limosus) baby on a U.S. quarter. (Photo by Brian Gratwicke, Smithsonian Conservation Biology Institute)

The limosa harlequin frog (Atelopus limosus), an endangered species native to Panama, now has a new lease on life. The Panama Amphibian Rescue and Conservation Project is successfully breeding the chevron-patterned form of the species in captivity for the first time. The rescue project is raising nine healthy frogs from one mating pair and hundreds of tadpoles from another pair.

“These frogs represent the last hope for their species,” said Brian Gratwicke, international coordinator for the project and a research biologist at the Smithsonian Conservation Biology Institute, one of six project partners. “This new generation is hugely inspiring to us as we work to conserve and care for this species and others.”

Nearly one-third of the world’s amphibian species are at risk of extinction. The rescue project aims to save priority species of frogs in Panama, one of the world’s last strongholds for amphibian biodiversity. While the global amphibian crisis is the result of habitat loss, climate change and pollution, a fungal disease, chytridiomycosis, is likely responsible for as many as 94 of 120 frog species disappearing since 1980.

Between its facilities at the Smithsonian Tropical Research Institute in Gamboa, Panama, and the El Valle Amphibian Conservation Center in El Valle, Panama, the rescue project currently cares for 55 adult limosa harlequin frogs of the chevron-patterned form and 10 of the plain-color form. The project has had limited success breeding the plain-color form of this species, and has successfully bred other challenging endangered species, including crowned treefrogs (Anotheca spinosa), horned marsupial frogs (Gastrotheca cornuta) and toad mountain harlequin frogs (A. certus).

Each species requires its own unique husbandry to thrive and breed. The project’s animal care team and scientists learn husbandry techniques as they work with a limited number of individuals. Jorge Guerrel, conservation biologist at the Smithsonian Tropical Research Institute, arranged rocks in the breeding tank to create the submerged caves that appear to be the preferred egg deposition sites for limosa harlequin frogs. Like other Atelopus species, tadpoles require highly oxygenated, gently flowing water between 22 and 24 degrees Celsius. The tadpoles’ natural food is algal film growing on submerged rocks, which Guerrel and his colleagues re-created by painting petri dishes with a solution of powdered spirulina algae, then allowing it to dry.

The mission of the Panama Amphibian Rescue and Conservation Project is to rescue amphibian species that are in extreme danger of extinction throughout Panama. The project’s efforts and expertise are focused on establishing assurance colonies and developing methodologies to reduce the impact of the amphibian chytrid fungus so that one day captive amphibians may be reintroduced to the wild. Current project partners include Cheyenne Mountain Zoo, Houston Zoo, Smithsonian’s National Zoological Park, Smithsonian Tropical Research Institute and Zoo New England.

Lindsay Renick Mayer, Smithsonian’s National Zoo

Cute in any form.

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Limosa harlequin frog (Atelopus limosus)

Highland color-form of the Limosa harlequin frog (Atelopus limosus)

Cute Frog of the Week: May 30, 2011

Camouflaged among the moss-covered rocks in the highlands and lowlands of Panama, the Limosa harlequin frog (Atelopus limosus) is difficult to spot even with its yellow clinging toes. These green-and-black-eyed amphibians leave their superb hiding places during the dry season and make their way to the fast-flowing streams of the Panamanian rainforest. Females lay their eggs in streambeds, where eager males wait to fertilize them. Laying clutches of eggs in faster-flowing waters may seem treacherous for the tiny frogs, but they brave the risk. Eggs that are laid in those areas of streams are less likely to be preyed upon, or to face competition from other frog species.

Limosa harlequin frog (Atelopus limosus)

Lowland color-form of the Limosa harlequin frog (Atelopus limosus)

Limosa harlequin frog tadpoles are not left to be swept away by hurried streams once they hatch. Suction disks on their bellies help anchor them to the rocks in the streambeds. However, the species seems to be losing its grip in its fight to survive. It is listed as endangered in the wild and the population is still decreasing. It is facing threats ranging from deforestation to pollution. Those threats are compounded by the ravaging affects of the chytrid fungus.  The Panama Amphibian Rescue and Conservation Project has brought the Limosa harlequin frog into captivity and is learning how to successfully breed them, giving hope for the survival of the species.

Photo credit: Brian Gratwicke, Panama Amphibian Rescue and Conservation Project

Every week the Panama Amphibian Rescue and Conservation Project posts a new photo of a cute frog from anywhere in the world with an interesting, fun and unique story to tell. Be sure to check back every Monday for the latest addition.

Send us your own cute frogs by uploading your photos here: http://www.flickr.com/groups/cutefrogoftheweek/

Notes from the Field: Finding the Limosa harlequin frog

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Jenyva Turner, Cheyenne Mountain Zoo animal keeper

Jenyva shows off an Atelopus limosus the Cheyenne Mountain Zoo team captured during the February expedition. (Photo courtesy of the Cheyenne Mountain Zoo)

Wanted: Adventurous expedition members to hike into the jungles of Panama looking for the rare Atelopus limosus. Must be willing to hike long hours in rugged, muddy terrain and in thigh-deep water, and ready to be wet, hungry, tired, and not afraid of spiders, snakes, scorpions, and lots of insects.

SIGN ME UP!!!

How could I pass up an opportunity to hike, explore, and camp in the jungle, all while helping save a species? This February was my first trip to Panama, however I followed the blog posts from other Cheyenne Mountain Zoo team members during previous expeditions to Panama, so I felt like I had already been there. I knew it was going to be a challenging trip, both physically and mentally. Would we find frogs? That was my biggest concern. As we all know, the chytrid fungus is taking its toll on frog populations around the world and the rare Atelopus limosus is not immune to chytrid’s deadly sting.

Atelopus limosus was our target species since the Panama Amphibian Rescue and Conservation Project only had one female and four males in captivity. The future of the species appeared to depend on our findings. It was the “dry season” in Panama (although, being from Colorado, rain every day does not seem very “dry”!) and therefore, was the best time to find females, as they would be coming down to the streambeds to lay their eggs. The males would be there waiting.

We hiked along the stream and carefully searched the moss-covered rocks for the small, highly camouflaged black and green frogs. It was tough to be quiet and sneak up on our target as we sloshed around streambeds, stepped over branches, and slipped on rocks. We paid especially close attention to areas of the stream where the water moved a little faster. Atelopus limosus lay their eggs in faster-moving water to reduce competition from other frog species and reduce predation. The tadpoles are specially designed with little suction cup disks on their bellies to help them hold onto the rocks and keep them from being washed away. Pretty cool!

Our team found just four Atelopus limosus during our time in the field, but we were able to give hope to the species as one of those caught was a young female. We handed off our precious cargo to the capable staff at the Summit Zoo, who take their job and role in frog conservation very seriously. They are working hard to care for and propagate many other amphibian species besides Atelopus limosus to ensure the sounds of frogs continue to be heard in the jungles of Panama.

Here’s a video of Antonia Chastain, a member of the Cheyenne Mountain Zoo team, finding out first-hand how difficult it is to catch an Atelopus limosus during the February trip:

—Jenyva Turner, Cheyenne Mountain Zoo animal keeper (and first-time frog finder!)

An update from Summit Zoo

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Toad Mountain harlequin frogs

A pair of the project's Toad Mountain harlequin frogs (Atelopus certus) were in amplexus for about 100 days and recently produced a clutch of eggs. (Photo by: Jorge Guerrel, Panama Amphibian Rescue and Conservation Project)

Hi amigos!

We are glad to give you the latest update on what is going on with our frogs here at the Panamanian Rescue and Conservation Project at the Summit Zoo in Panama. And we are going to start with some great news: After almost 100 days of a very long amplexus (from the latin “embrace,” amplexus is a form of pseudocopulation in which a male amphibian grasps a female with his front legs as part of the mating process), we have our very first Toad Mountain harlequin frog (Atelopus certus) clutch!

This is huge news especially since A. certus is facing a very high risk of extinction in the wild and is classified as “endangered” by the IUCN Red List of Threatened Species. The Toad Mountain harlequin frog is an endemic species from the Darien region of eastern Panama and little is known about its reproductive and breeding behavior. From observations made here at the Summit Zoo in Panama, we have noticed some interesting behavior. For example, during amplexus, the male A. certus holds on to the female so tight that he won’t eat for three months or more. We are taking notes and paying attention to the smallest change in water quality and temperature in their tank to assure the largest number of juveniles possible.

La loma tree frog morph

The rescue project is the first ever to successfully breed the La loma tree frog. (Photo by: Jorge Gurrel)

The rest of the group is doing just fine. The La loma treefrog (Hyloscirtus colymba) tadpoles are growing and some have fully developed legs, though we still need to wait until they come out of the water and absorb the tail to place them in their new individual tanks. The baby Limosa harlequin frogs (Atelopus limosus) are bigger and stronger–they have been eating lots of springtails and we are making sure that UV light is always available to them to prevent any bone disease.

The male adults are calling very often, especially early in the morning for our diurnal species, such as the Pirre harlequin frog (Atelopus glyphus). The rest of the harlequin frogs, H. colymba and our single male Bob’s robber frog (Craugastor punctariolus) call to attract their females throughout the night, particularly when is raining. We are also testing a few ways to feed the big C. punctariolus so we can offer them a variety of food as part of their diet.

Thanks to our collaborators and volunteers for all their suggestions and new ideas.

That’s all for now, but we will continue to keep you updated. Thanks for your support!

-Angie Estrada, Panama Amphibian Rescue and Conservation Project

Rescue Project First to Breed Critically Endangered Tree Frog

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Atelopus limosus

The Panama Amphibian Rescue and Conservation Project has successfully bred the endangered Limosa harlequin frog, Atelopus limosus. (Credit: Angie Estrada, Panama Amphibian Rescue and Conservation Project)

As frogs around the world continue to disappear—many killed by a rapidly spreading disease called chytridiomycosis, which attacks the skin cells of amphibians—one critically endangered species has received an encouraging boost. Although the La Loma tree frog, Hyloscirtus colymba, is notoriously difficult to care for in captivity, the Panama Amphibian Rescue and Conservation Project is the first to successfully breed this species.

“We are some of the first researchers to attempt to breed these animals into captivity and we have very little information about how to care for them,” said Brian Gratwicke, international coordinator for the project and a research biologist at the Smithsonian’s National Zoo, one of nine project partners. “We were warned that we might not be able to keep these frogs alive, but through a little bit of guesswork, attention to detail and collaboration with other husbandry experts—we’ve managed to breed them. The lessons we’re learning have put us on target to save this incredible species and our other priority species in Panama.”

The rescue project currently has 28 adult La Loma tree frogs and four tadpoles at the Summit Municipal Park outside of Panama City, Panama. In addition to the La Loma tree frog, the project also has successfully bred the endangered Limosa harlequin frog, Atelopus limosus. Keepers will continue to carefully monitor the tadpoles of both species.

H. colymba

Although the La Loma tree frog, Hyloscirtus colymba, is notoriously difficult to care for in captivity, the rescue project is the first to successfully breed this species. (Credit: Brian Gratwice, Smithsonian's National Zoo)

Nearly one-third of the world’s amphibian species are at risk of extinction. The rescue project aims to save more than 20 species of frogs in Panama, one of the world’s last strongholds for amphibian biodiversity. While the global amphibian crisis is the result of habitat loss, climate change and pollution, chytridiomycosis is likely at least partly responsible for the disappearances of 94 of the 120 frog species thought to have gone extinct since 1980.

“Although the outlook for amphibians is grim, the rescue project’s recent developments give us hope for these unique Panamanian species,” said Roberto Ibáñez, local director of the project and a scientist at the Smithsonian Tropical Research Institute, one of the project’s partners. “We are creating what amounts to an ark for these animals so that their species may survive this deadly disease. We’re also looking for a cure so that someday we can safely release the frogs back into the wild.”

Of Panama’s six harlequin frog species, five are in collections at the Summit Zoological Park and the El Valle Amphibian Conservation Center in El Valle, Panama. One species, the Chiriqui harlequin frog, A. chiriquiensis, from western Panama, is likely extinct. The other species range from being extinct in the wild—the Panamanian golden frog, A. zeteki—to being endangered.

The mission of the Panama Amphibian Rescue and Conservation Project is to rescue amphibian species that are in extreme danger of extinction throughout Panama. The project’s efforts and expertise are focused on establishing assurance colonies and developing methodologies to reduce the impact of the amphibian chytrid fungus so that one day captive amphibians may be reintroduced to the wild. Project participants include Africam Safari, Panama’s Autoridad Nacional del Ambiente, Cheyenne Mountain Zoo, Defenders of Wildlife, El Valle Amphibian Conservation Center, Houston Zoo, Smithsonian’s National Zoological Park, Smithsonian Tropical Research Institute, Summit Municipal Park and Zoo New England.

# # #

Media only: contact Lindsay Renick Mayer, Smithsonian’s National Zoo, 202-633-3081

Guppy Travels: Day Three

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Harlequin frog (Atelopus limosus)

This female harlequin frog (Atleopus limosus) is the only female of her kind (the highland variation) that the rescue project has in captivity.

There’s something almost sublime about her and it’s not just the way we angle the light during her photo shoots. She’s of the highland variation of Atelopus limosus, a harlequin frog from Cerro Brewster, and she happens to be the only female of her kind that we’ve got in captivity.

Talk about pressure.

Before I left for Panama, I already knew about her and to be honest, to be perfectly honest, she was the frog at Summit Zoo I was most looking forward to meeting. I’m not sure what I expected. She’s certainly a beauty, but in my opinion, most frogs are. Did I expect her to indicate, in some way, that she understands the significance of her position in the Universe? And if I did, what did I expect that sign to look like? A knowing nod? A regal posture upon a bromeliad? An extra quick flick of the tongue?

What I do know is that every time I’ve had to open her tank over the last few days, to clean it or to take her photo, my heart has started racing. I imagine her escaping, getting hurt or getting lost, and taking with her the possibility that the rescue project will be able to save these dark brown frogs with striking green chevrons. The frog keepers at Summit Zoo must feel the weight of this responsibility every day with every frog in their care. I’m not sure I could handle the gravity of that responsibility with the same level of grace that I’ve seen in them.

The reality, of course, is that one female isn’t going to be enough to build a genetically diverse population of these frogs. She was one of the frogs the project collected last year from Cerro Brewster in Panama’s Chagres National Park, where chytrid had spread rapidly, surprising (and, I think, momentarily devastating) our researchers who had hoped to beat the wave of the disease there. We haven’t stopped the search and we hope to find more females to add to our ark early next year.

Atelopus limosus tadpoles

The project's first group of tadpoles belongs to the lowland variation of Atelopus limosus.

Hope is really what drives the project. And really, how can it not? The situation may be dire, but there’s a song of hope in the call of one of the project’s male Harlequin frogs of the lowland variation in a tank in the middle of the rescue pod. There’s hope in the adorable adolescent Toad Mountain harlequin frogs (Atelopus certus) in tanks at the front of the pod. There’s hope in the far end of the rescue pod where a large tank holds what may be hundreds of tadpoles—a first for the rescue project. The tadpoles are not the highland variation of the harlequin frog, but the lowland variation, which is less threatened than their more colorful counterparts. Still, each step toward successful breeding marks a victory for us and provides an encouraging boost.

And perhaps that is what accounts for the sole female Harlequin frog’s seemingly ethereal beauty: in part because of her, we still have plenty of hope.

—Lindsay Renick Mayer, Smithsonian’s National Zoo

Press release: Panama amphibian rescue challenged by rapid spread of chytrid fungus

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The Panama Amphibian Rescue and Conservation Project announced on December 4th, 2009  that some of the frogs collected in their inaugural expedition in November were already affected by amphibian chytrid fungus (Batrachochytrium dendrobatidis), the disease that has devastated nearly 100 frog species worldwide.

Hyloscirtus colymba undergoing treatment (c) Matt Evans NZP

Hyloscirtus colymba undergoing treatment Photo: (c) Matt Evans NZP

The purpose of the Project’s first expedition was to collect living specimens of frogs not yet affected by chytrid fungus in Panama for captive breeding. The rescue mission, led by the Dr. Roberto Ibanez and Edgardo Griffith with support from all of the partners, collected approximately 20 harlequin toads (Atelopus limosus) and 20 treefrogs (Hyloscirtus colymba) under brutal field conditions at the peak of the rainy season from Cerro Brewster in Panama’s Chagres National Park.

These two species will be kept at the Summit Municipal Park in Panama while other rescued species were taken to the previously established El Valle Amphibian Conservation Center (EVACC). The EVACC was established in 2005 ago by the Houston Zoo and many other zoo partners in response to massive amphibian declines were witnessed in Western Panama by researchers and visiting scientists at the Smithsonian Tropical Research Institute.

When the animals arrived at the rescue facility, several of the frogs were already showing signs of illness and many more developed signs in the following days. Investigation by the project veterinarians identified these animals to be infected with the amphibian chytrid fungus. Samples were further examined by Dr. Roberto Ibanez at the Smithsonian Tropical Research Institute and the chytrid fungus was confirmed.

Eric Baitchman of Zoo New England treating Atelopus limosus (c) Matt Evans

Eric Baitchman of Zoo New England treating Atelopus limosus Photo: (c) Matt Evans

“Looking through the microscope and seeing the chytrid organism present on these animals with my own eyes was devastating. I knew this meant we were already behind. This meant that the animals we were treating could very well be the last members of their species unless we act fast to get back out there and save as many more as we can from the wild,” said Dr. Eric Baitchman, Director of Veterinary Services at Zoo New England.

All animals collected began a 10-day treatment protocol to eliminate the fungus, which involves each animal being bathed in a medicated solution for 10 minutes a day. This is a standard protocol for animals that are collected from the wild, due to the risk of the amphibian chytrid pathogen, though this treatment was all the more vital once the organism was actually identified in these animals. Animals that are actively showing signs of illness also received intensive supportive care to help them survive through the course of treatment. The amphibian chytrid  attacks the skin cells of amphibians, which can be quickly lethal for an animal that relies on its skin for the majority of respiratory function, hydration and electrolyte balance. Veterinary care for afflicted animals includes continuous fluid therapy to maintain hydration and replace electrolytes and antibiotic treatment to protect against other infections that may take hold after the loss of the skin’s protective barrier.

The worst affected animals were unfortunately lost, but many animals are improved and are recovering today.

The discovery of amphibian chytrid on the frogs at this site means that time has nearly expired for at least four species of frogs that do not live further east than Cerro Brewster and have been extirpated at all their other known sites.

“If we want to save these species, we will have to ramp up capacity a lot faster than anticipated,” said Brian Gratwicke of the Smithsonian’s National Zoo. “We thought we had some breathing space, but in fact, we may already be too late for some species.”

Responding to this news, the Autoridad Nacional del Ambiente (ANAM), Panama’s National Environmental Authority, announced it would join the Project, pledging $150,000 in support.

“Amphibians are an important part of the cultural and biodiversity heritage of Panama,” said Javier Arias, director of ANAM. “We are pleased to announce our financial and logistical support as full partners in the Panama Amphibian Rescue and Conservation Project to build Panamanian capacity to respond to the global amphibian decline crisis.”

The founding partners of the Project are Cheyenne Mountain Zoo, the Smithsonian’s National Zoo, Houston Zoo, Africam Safari in Mexico, Zoo New England, the Summit Municipal Park in Panama, Defenders of Wildlife and the Smithsonian Tropical Research Institute. These research and conservation organizations came together earlier in the year and pledged their support to combat the amphibian crisis in Panama.

The project partners are collectively working to establish an additional amphibian conservation breeding center in Panama for critically endangered species that are in jeopardy of being eradicated by the chytrid fungus. Houston Zoo and other zoo partners created a facility dedicated mainly to preserve endangered species of amphibian from Central Panama known as EVACC, a project lead by the Panamanian biologist Edgardo Griffith in 2005, and the additional capacity provided by this project is needed to focus on Eastern Panama.

The Association of Zoos and Aquariums has challenged all AZA accredited Zoos and Aquariums to take a leadership role during this amphibian crisis. If this effort fails, one-third to one-half of the world’s amphibians could go extinct.